Accepted_test

The naked mole rat (Heterocephalus glaber) serves as an excellent model for studying genome stability due to its exceptional lifespan compared to similarly sized mice. Its cells exhibit unique genomic, transcriptomic, and proteomic features, contributing to their resistance to aging, cancer, and oxidative stress, alongside more efficient DNA repair mechanisms. Central to these repair processes is the enzyme PARP1, which regulates DNA repair through poly(ADP-ribosyl)ation. Previous studies indicated significantly lower PARP1 automodification in naked mole rat cells compared to human cells, possibly due to differences in PARP1 concentration and protein factors. This research aims to produce recombinant PARP1 from both species to compare their kinetic parameters, DNA affinity, thermal stability, and resistance to denaturation. This study highlights the potential mechanisms underlying the naked mole rat's remarkable longevity by examining the biochemical properties of its PARP1 enzyme.