Accepted_test

The approaches for CRISPR/Cas9 regulation on the guide RNA level
by Novopashina Darya | Sakovina Lubov | Gorlenko Elensa | Dolzhikova Olga | Meschaninova Mariya | Institute of Chemical Biology and Fundamental Medicine SB RAS | Institute of Chemical Biology and Fundamental Medicine SB RAS | Institute of Chemical Biology and Fundamental Medicine SB RAS | Institute of Chemical Biology and Fundamental Medicine SB RAS | Institute of Chemical Biology and Fundamental Medicine SB RAS
Abstract ID: 220
Event: BGRS-abstracts
Sections: [Sym 9] Section “Gene and genome editing in modeling human pathological disease processes”

The increase of effectivity and accuracy of gene editing system is the actual task of modern molecular biology and gene engineering. Design of regulated CRISPR/Cas systems and development of approaches for controlling their activity by physico-chemical stimuli are especially interesting in the context of gene editing. Among such approaches, activity regulation on the level of guide RNAs by introducing chemical modifications, changing the sequences or adding the blocking oligonucleotide are seem to be very promising. The aim of this research is design and testing of CRISPR/Cas9 system that can be regulated by irradiation and small molecules.  We proposed a set of approaches for design of CRISPR/Cas9 systems which can be regulated by irradiation or by small molecules.