Accepted_test

Absence of 5mC in thermo- tolerant yeast and telomere-to-telomere assembly by nanopore technology

by Andrey Eremin | Lomonosov Moscow State University

Abstract ID: 225

Event: BGRS-abstracts

Sections: [Sym 1] Section “Structural-functional organization of genomes and transcriptomics”

The abstract presents a study on the thermotolerant methylotrophic yeast, Ogataea parapolymorpha DL-1, utilizing long-read sequencing technology for a comprehensive analysis of its genome and methylome. This yeast species is noted for its biotechnological applications, particularly in protein production and methanol metabolism studies.

The main finding describes a rare methylation pattern - Ogataea parapolymorpha DL-1 does not have 5mC modifications in its genome at all, but has 6mA instead, uniformly distributed across genome. Authors also report a new DNA motif that is connected to 6mA modification.

As a second result, authors introduce telomere-to-telomere assembly which closes gaps, corrects assembly mistakes in the existing draft created with short-read technology, and describes new genomic features.