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Wheat Triticum aestivum L. is one of the major cereal crops on the globe. The number of wheat varieties and subspecies is enormous and continues to increase. Metabolic studies provide a deeper understanding of phenotypic diversity within the species polymorphism of T. aestivum and are key to chemotaxonomic classification and identification of species and subspecies based on their chemical composition. Determination of the chemical composition of lipophilic metabolites in green leaf extracts will provide an opportunity to establish relationships both within individual metabolic pathways and between these specific pathways. The realization of such a comparative study on a wide sample of T. aestivum cultivars and subspecies is the aim of this study.