Accepted_test

The aim of this work was to study the effect of graphene and graphene oxide on the stress resistance of Escherichia coli bacteria and molecular mechanisms of the key DNA-binding protein Dps that ensures stress resistance of bacteria. For this purpose, microbiological and molecular dynamics studies were carried out.

It was found that the growth of E. coli bacteria in the presence of G and GO leads to a sharp decrease in their stress resistance to temperature and UV exposure. As a result of molecular dynamics simulations, the influence of G and GO on dynamics and structure of DNA- Dps was revealed. It has been shown that graphene can lead to changes in the quaternary structure of the protein. DNA in complexes tends to contact both the protein and the substrate simultaneously, which leads to structural rearrangements or destruction of Dps-DNA complexes.

The data obtained are of practical interest for the development of new sterilization methods and for researchers of the structure of biological molecules and their complexes on the surface of graphene substrates. The research results can be used to create nanomaterials based on biological molecules with desired properties.