Accepted_test

The global scientific community is conducting extensive research to determine the degree of influence of the human gut microbiota on the outcomes of immunotherapy for malignant tumors. However, the identification of candidate bacteria is complicated by the location and limitations of the samples collected, as well as some other objective factors. To overcome these limitations, a meta-analysis was performed using gut metagenomes and advanced bioinformatics techniques. According to the analysis results, our study uncovered significant findings that deepen the understanding of the intricate relationship between gut microbes and the efficacy of melanoma immunotherapy. In particular, we discovered the specific metagenomic profile of patients with favorable treatment outcomes, characterized by a prevalence of MAGs with increased overall metabolic potential and proficiency in polysaccharide utilization, along with those responsible for cobalamin and amino acid production. Furthermore, our investigation of the biosynthetic pathways of short-chain fatty acids, known for their immunomodulatory role, revealed a differential abundance of these pathways among the specific MAGs. Among others, the cobalamin-dependent Wood-Ljungdahl pathway of acetate synthesis was directly associated with responsiveness to melanoma immunotherapy.