Accepted_test

Transcriptomic response of genes related to the glutamate- and GABA-ergic systems in the hypothalamus of ISIAH and WAG rats exposed to acute restraint stress
by Plekanchuk V.S. | Ryazanova M.A. | Oshchepkov D.Yu. | Redina O.E. | Markel A.L. | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia Kurchatov Genomic Center ICiG SB RAS, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, Russia
Abstract ID: 324
Event: BGRS-abstracts
Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

Glutamate and GABA are the primary neurotransmitters that provide the necessary level of excitation of the hypothalamic neuroendocrine cells. An imbalance of these neurotransmitters can lead to significant changes in HPA axis activity [1]. The aim of the work was to compare changes in the transcriptional activity of genes related to the glutamate- and GABA-ergic systems of the hypothalamus in response to acute restriction stress in ISIAH rats modeling a stress-sensitive form of arterial hypertension and in normotensive WAG rats. Downregulation of the expression of the Gad2, Grik3 and Gabrb3 genes was shown in rats of both strains and, accordingly, does not depend on the genotype. The strain-specific response to stress in the hypothalamus of hypertensive ISIAH rats is associated with a decrease in the transcription of the Grm5 and Gabrb2 genes, and in the hypothalamus of normotensive WAG rats - with a change in the level of transcription of the Gabrq and Gabrd genes. The identified interstrain differences may modulate the activity of synaptic transmission in the hypothalamus and contribute to different stress reactivity in normotensive WAG and ISIAH rats, which are an animal model a stress-sensitive form of arterial hypertension.