Accepted_test

Genomic technologies in the study of the phylogeny of plague pathogen and certification of foci of the Caspian region and Central Asia
by Alina N. Balykova | Alexander V. Kovrizhnikov | Galina A. Eroshenko | Yaroslav M. Krasnov | Vladimir V. Kutyrev | Russian Research Anti-Plague Institute “Microbe” | Russian Research Anti-Plague Institute “Microbe” | Russian Research Anti-Plague Institute “Microbe” | Russian Research Anti-Plague Institute “Microbe” | Russian Research Anti-Plague Institute “Microbe”
Abstract ID: 334
Event: BGRS-abstracts
Sections: [Sym 5] Section “Molecular phylogenetics and phylogenomics of Plants, Fungi, Protists, Prokaryotes and Viruses”

Based on the results of comparative and phylogenetic analyses of 350 strains of Yersinia pestis isolated in 1912-2015 in 25 natural plague foci in four countries: Russia, Kazakhstan, Turkmenistan and Uzbekistan, new data on the evolution, distribution of phylogroups and genetic variability of populations of the highly virulent and epidemically significant medieval biovar of the plague pathogen widespread in Russia and the CIS countries were obtained. These data are important for reconstruction of the spatial and temporal circulation of the medieval biovar during the epidemic activity of plague foci in the Caspian region and for establishing the reasons for further expansion of the pathogen in Eastern Europe and Central Asia. SNP-/VNTR-/CRISPR-loci of the genome of the medieval biovar were analyzed using the author's computer programs. 59 SNPs were identified and SNP panels are being developed for molecular genetic certification of plague foci. The profile of CRISPR elements and spacers (YPa a1-a2-a3; YPb b1-b2-b3-b4`; YPc c1-c2-c3) was established; variant VNTR loci providing differentiation of genetic populations of Y. pestis of medieval biovar of different origin were determined. The obtained results are important for the study of variability of the genome architecture of the plague pathogen and for improving the efficiency of molecular epidemiologic tracing of the origin of pathogenic strains during molecular epidemiologic monitoring of plague foci.