Accepted_test

Features of the secondary structure of the ITS2 rRNA spacer region of trematodes from the superfamily Echinostomatoidea
by Kalinina K.A. | Tatonova Y.V. | Shchelkanov M.Y. | 1.G.P. Somov Research Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Vladivostok, Russia. 2.Federal Scientific Center for Biodiversity of Terrestrial Biota of East Asia, Far Eastern Branch of the Russian Academy of Sciences, Academy of Sciences, Vladivostok, Russia | 1.G.P. Somov Research Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Vladivostok, Russia. 2.Federal Scientific Center for Biodiversity of Terrestrial Biota of East Asia, Far Eastern Branch of the Russian Academy of Sciences, Academy of Sciences, Vladivostok, Russia | 1.G.P. Somov Research Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Vladivostok, Russia. 2.Federal Scientific Center for Biodiversity of Terrestrial Biota of East Asia, Far Eastern Branch of the Russian Academy of Sciences, Academy of Sciences, Vladivostok, Russia
Abstract ID: 338
Event: BGRS-abstracts
Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

The ITS2 rRNA spacer region were obtained for previously described trematodes: Echinochasmus pseudobeleocephalus (2023), Sphaeridiotrema ussuriensis, S. aziaticus,
S. pyriforme, and Psilotrema limosum (2022). Sequence analysis of ITS2 was performed to see if there was interspecies variation. Phylogenetic analysis of the full sequence ITS2 was performed using Bayesian inference (BI) and maximum likelihood (ML) algorithms. The work compared ITS2 sequences from 66 species. These species cover 7 families: Echinostomatidae, Echinochasmidae, Psilostomatidae, Fasciolidae, Eucotylidae, Philophthalmidae, Cyclocoelidae. The sequences vary in length from 235 to 1002 bp. Secondary structure folding predicted a four-domain model with only the A and B domains conserved. The phylogenetic tree based on the sequences of the ITS2 rDNA region is similar to previously obtained data on the 28S rRNA gene by Tkach and co-authors (2016).