Accepted_test

Motivation and Aim: Obesity is a complex, multifactorial disease characterized by
excessive fat accumulation that may impair health. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play an important role in adipogenesis, lipid metabolism, and insulin response.  Our research investigates the differential expression of a wide range of lncRNAs (ASMER1, SNHG9, P5549, p19461, GAS5 and MALAT1) and miRNAs (MIR26A1, MIR222, MIR221 and MIR155) in the visceral adipose tissues of individuals with and without abdominal obesity.
Methods and Algorithms:
The study included a case group with abdominal obesity and a control group without obesity, in total 100 participants. White visceral adipose tissue samples were collected from individuals during elective surgery.
RNA was extracted and Quantitative PCR (qPCR) was performed using SYBR dye to
determine the relative expression levels of targeted microRNAs and lncRNAs.
Correlations between RNA levels and various clinical indicators were determined using Student t-tests and Mann–Whitney U tests.

Results: Expression of ASMER1 and MALAT1 as well as miR222 and miR221 was undetectable in the homogenate of visceral fat tissue. RNA levels of P5549 and P19461 were at the borderline of detection levels and showed no reliable differences between the control and case groups. Expression levels of SNHG9, GAS5, and miR155 showed no significant difference between participants with and without obesity, while the expression of miR26A1 was significantly lower in the case group.
Conclusion:
Our study is the first to show that miR26A is significantly downregulated in the
visceral adipose tissue of obese individuals.