Accepted_test

Splicing isoforms of SIRT1 in the liver of patients with metabolic syndrome - expectation/reality
by Gorbacheva Anna Mikhailovna | Voronova Sophia Sergeevna | Bograya Maria Mikhailovna | Woolf Maria Alexandrovna | Litvinova Larisa Sergeevna | IKBFU | IKBFU | IKBFU | IKBFU | IKBFU
Abstract ID: 361
Event: BGRS-abstracts
Sections: [Sym 9] Section “Gene expression and human diseases”

SIRT1 has been extensively studied in context of metabolic syndrome (MetS), but its isoforms remain largely unexplored. SIRT1 isoforms are generated by alternative splicing: isoform 1 (V1) has two nuclear localization signals, while the other two isoforms (V2 and V3) are localized in the cytoplasm. Therefore, the aim of current study was to perform a bioinformatic prediction of the protein-protein interactions (PPIs) by PEPPI tool between the SIRT1 isoforms and the transcription factors PGC-1α, PPAR-α and PPAR-γ and to investigate the expression levels of the SIRT1 isoforms and the listed transcription factors in the liver of patients with MetS. We suggest that isoform V1 deacetylates the histones of PPAR-α and PPAR-γ promoters in the cell nucleus, whereas V2 and V3 directly interact with PGC-1α and PPAR-α in the cytoplasm. The ratio of mRNA levels of SIRT1 isoforms in the liver did not differ between the control group and the group with MetS. However, the expression of V3 was higher in patients with MetS. Thus, SIRT1 isoforms may differentially modulate mitochondrial biogenesis, lipogenesis and lipolysis, indicating the need for further research in this area.