Accepted_test

A Novel Environmental Pseudomonas Jumbo phage Pse-uM_296: genome analysis and putative taxonomy
by Morozova V.V. | Mogileva A.A. | Kozlova Yu.N. | Tikunov A.Yu. | Fedorets V.A. | Yakubovskij V.I. | Zhirakovskaya E.V. | Tikunova N.V. | ICBFM SB RAS, Novosibirsk, Russia | ICBFM SB RAS; Novosibirsk State University, Novosibirsk, Russia | ICBFM SB RAS, Novosibirsk, Russia | ICBFM SB RAS, Novosibirsk, Russia | ICBFM SB RAS; Novosibirsk State University, Novosibirsk, Russia | ICBFM SB RAS; Novosibirsk State University, Novosibirsk, Russia | ICBFM SB RAS, Novosibirsk, Russia | ICBFM SB RAS, Novosibirsk, Russia
Abstract ID: 376
Event: BGRS-abstracts
Sections: [Sym 5] Section “Molecular phylogenetics and phylogenomics of Plants, Fungi, Protists, Prokaryotes and Viruses”

The representatives of the genus Pseudomonas encode a huge variety of metabolic pathways and can be used in biotechnology, agriculture and remediation. The vast majority of known Pseudomonas phages are specific to the nosocomial pathogen Pseudomonas aeruginosa. As the environmental Pseudomonas phages can affect pseudomonads, so these phages need to be studied. The phage PseuM_296 and its bacterial host Pseudomonas mandelii were isolated from the water sample. Electron microscopy revealed that PseuM_296 virions have myovirus morphotype. The PseuM_296 had a large genome, 273 174 bp in length; it contained 332 putative genes and three of them correspond to tRNAs. Only 81 genes encode proteins with predicted functions, and the remaining 248 genes were defined as hypothetical. Genes encoding two multisubunit RNAPs, SbcCD complex ATPase, DnaB-like helicase, RNA helicase, and family B DNA polymerase were revealed in the genome. These genes are defined as the core ones for the phiKZ-like group of jumbo phages. Phylogenetic proteomic analysis сonfirmed that phage PseuM_296 belongs to the group of phiKZ-like phages. Intergenomic similarity analysis revealed that PseuM_296 may be a prototype species of a new genus in the phiKZ-like group of giant bacteriophages.