Accepted_test

Determination of the genetic signature of chemically polarized rat macrophages

by Viktoriia Kiseleva | Federal State Budget Institution «National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov» Ministry of Healthcare of the Russian Federation, 4 Oparina Street, 117997 Moscow, Russian Federation

Abstract ID: 378

Event: BGRS-abstracts

Sections: [Sym 1] Section “Genome-wide transcriptomics (differential gene expression)”

Macrophages, the cells of the innate immune response, can exhibit two opposite states, pro-inflammatory and anti-inflammatory, depending on the surrounding conditions. In the era of personalized medicine and the development of cellular technologies, they are of great interest as they play a significant role in the pathogenesis of many diseases. Studies of the role and potential therapeutic effects of macrophages are often carried out on animal models, which are the bridge between science and the clinic. And if the immune processes of the mouse are described in quite detail, there are still many white spots for the rat. Thus, in the literature there are single works devoted to the study of mechanisms of polarization of bone marrow-derived macrophages at the transcriptional level. The aim of this work was to determine the genetic signatures of rat macrophages polarized in vitro by proinflammatory and anti-inflammatory stimuli. As a result, the genetic signature of macrophages with proinflammatory and anti-inflammatory phenotype was determined. The most activated signalling pathways under LPS and IL-4/IL-10 stimulation were identified.