Accepted_test

ADP-ribosylation is a posttranslational modification of proteins that plays a key role in many regulatory mechanisms and cellular processes. Previously, it was believed that ADP-ribosylation occurs mainly during DNA repair, but recently a lot of data has been obtained on the participation of PARP enzymes in a variety of cellular processes, in particular, in the regulation of ribosome biogenesis and gene expression at the translation level. For the first time, we found out which enzymes and under what conditions ADP-ribosylated human ribosomal proteins. It was shown for the first time that the reaction depends on the simultaneous presence of a DNA activator and a HPF1 cofactor, and is completely inhibited when the concentration of ribosomal proteins reaches a certain threshold value.