Accepted_test

Viruses cause serious illness throughout the world, especially in developing countries. As populations grow in size, density, and mobility, the risk of outbreaks of known and unknown viral infections increases. In recent years, using NGS technology, many new tick-borne viruses pathogenic to humans have been identified, including orthonairoviruses: Songling virus (SGLV), Yezo virus (YEZV) and Beiji nairovirus (BJNV). The limited data available on these viruses indicate that they are highly likely to cause fever in humans, representing an emerging public health threat. At the same time, information about the structural features of the virion and proteins of new orthonairoviruses is currently completely absent. In the course of this study, based on computational models, the structural features of the nucleoproteins of three new orthonairoviruses were identified, and approaches were developed for the experimental study of the structure of recombinant proteins of poorly studied viruses that are potentially pathogenic for humans. Further study of the protein structure of new viruses will provide insight into the fundamental mechanisms of infections caused by these viruses and will allow for a targeted development of effective vaccines and antiviral drugs to ensure national biological safety.