Accepted_test

Phosphorylguanidine derivatives of oligonucleotide as perspective tools for development of highly sensitive systems for nucleic acids diagnostics
by Dmitrienko E. | Bulgakova A. | Chubarov A. | Lomzov A. | Pyshnyi D. | Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia | Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia | Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia | Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia | Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia
Abstract ID: 454
Event: BGRS-abstracts
Sections: [Sym 9] Section “Molecular Pathology, Diagnostics, and Therapeutics”

Nucleic acids (NA), their analogues and derivatives are molecular tools for scientific molecular biological research and are also used to solve practical problems in biomedicine, biotechnology and molecular diagnostics. The isolation of nucleic acids from biological samples and their analysis by various methods represents one of the most significant problems of global health, as it is essential for clinical diagnosis and detection of pathologies and pathogens of socially important infections. However, the low efficiency and specificity of NA isolation significantly complicate further analysis and lead to false negatives or false positives.

Introduction of chemical modifications into the composition of oligonucleotides allows to change their structure, properties, and interaction with biologically active compounds. Synthetic analogues of nucleic acids often have improved physicochemical, molecular-biological and biological properties. The use of modified oligonucleotides, construction of diagnostic and therapeutic systems based on them, as well as their use in various heterophase formats for efficient isolation and analysis allows solving the problems of modern NA diagnostics.

We have obtained a set of data that further reveal the potential of using phosphorylguanidine oligonucleotides for the development of modern nucleic acid analysis systems, and developed algorithms for the directed design of highly specific probes and primers to solve the most complex applied problems in the field of molecular diagnostics of NA.