Accepted_test

Features of the interaction of piRNA with mRNA of the FGF2 gene
by Anatoliy Ivashchenko | Anna Pyrkova | Aigul Akimniyazova | Timur Kerimov | Igor Yershov | Sultanali Sultanbekov | al-Farabi Kazakh National University | al-Farabi Kazakh National University | al-Farabi Kazakh National University | al-Farabi Kazakh National University | al-Farabi Kazakh National University | al-Farabi Kazakh National University
Abstract ID: 46
Event: BGRS-abstracts
Sections: [Sym 11] Section “Transcription, splicing, translation”

This study investigates the involvement of piRNAs in regulating the expression of the fibroblast growth factor 2 (FGF2) gene, a crucial player in various diseases. Employing the MirTarget program, binding sites of piRNAs on FGF2 mRNA were identified, focusing on the 5'UTR, CDS, and 3'UTR regions. Results reveal selective binding of 219 piRNAs to the 3'UTR of FGF2 mRNA, forming canonical (A-U, G-C) and non-canonical (A-C, G-U) nucleotide pairs. Notably, piR-618251 displayed strong interaction with FGF2 mRNA, exhibiting a ΔG/ΔGm value of 94%. Analysis demonstrates a compact distribution of piRNA binding sites within a small region of FGF2 mRNA, suggesting competitive binding among piRNAs. Furthermore, piRNA clusters were observed, indicating potential competition for mRNA binding. These findings imply a regulatory role of piRNAs in suppressing FGF2 mRNA translation. However, the abundance of piRNAs targeting a single gene and their compact localization on mRNA warrant further investigation. This study underscores the complexity of piRNA-mediated gene regulation and calls for comprehensive exploration of similar associations between miRNAs, piRNAs, and mRNA. Overall, piRNAs emerge as potential modulators of FGF2 gene expression, offering insights into novel regulatory mechanisms in disease pathogenesis.