Accepted_test

Genomics and physiology of filamentous colorless sulfur bacteria
by Grabovich M.Yu. | Rudenko T.S. | Smolyakov D.D. | Ravin N.V. | Voronezh State University, Voronezh, Russia | Voronezh State University, Voronezh, Russia | Voronezh State University, Voronezh, Russia | Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia
Abstract ID: 495
Event: BGRS-abstracts
Sections: [Sym 8] Section “Microbial communities of natural and anthropogenic habitats”

Colorless sulfur bacteria are one group of lithotrophic prokaryotes that dominate natural habitats with high hydrogen sulfide concentration. It forms powerful mats and fouling visible to the naked eye and accumulates sulfur inside the cells. The absence of reliable phylogenetic markers and the small number of isolates prevent the establishment of a precise phylogenetic relationship in the group of colorless sulfur bacteria. Long-term studies on the genetic basis of natural diversity, physiology and taxonomy of one of the groups of colorless sulfur bacteria of the genus Thiothrix are summarized. A full-genome phylogeny of the genus Thiothrix, based on a wide sample of sequenced genomes, was established. Using phylogenetic analysis based on full-genome sequences, the genus Thiothrix was expanded to 15 species, for 9 of which we determined genome sequences. A new genus within the family Thiothrichaceae 'Candidatus Thiocaldithrix' gen. nov. was also described. Pangenomic analysis of the genus Thiothrix was carried out. Phylogenetic markers: tRNA(Ile)-lysidine synthase (tilS) and β-subunit of RNA polymerase (rpoB), that can be used for rapid analysis of pure cultures and natural communities for phylogenetic identification of representatives of the genus Thiothrix, were found. Based on full-genome analysis, an updated phylogeny was established for the family Beggiatoaceae, particularly for the genera 'Candidatus Parabeggiatoa' and 'Candidatus Albibeggiatoa', and clarity was brought to the understanding of the metabolic potential of colorless sulfur bacteria from these genera.