Accepted_test

Well-established bacterial typing approaches, including 23S RNA sequencing or multilocus typing, may be insufficient for resolution of different close strains and lack insight into metabolic capabilities of these preparations. In this work, we tested the applicability of a proteogenomic approach for metabolic pathway mapping in Lactococcus lactis. Six draft genomes were obtained and annotated from the sequencing data.  All isolates were typed as Lactococcus lactis subsp. lactis bv. diacetylactis strain SD96. Each of the samples is characterized with a shotgun proteomic data in multiple biological replicates. It was not possible to conclusively distinguish samples by characteristic protein signatures or metabolic pathways, but it is still possible to infer possible pathways in the culture of interest.