Accepted_test

Local structure of basal DNA promoters in eukaryotic tRNA genes

by Savina Ekaterina | I.M. Sechenov First Moscow State Medical University of the Russian Ministry of Health (Sechenov University), Moscow, Russia

Abstract ID: 501

Event: BGRS-abstracts

Sections: [Sym 1] Section “Structural-functional organization of genomes and transcriptomics”

Transcription of all types RNA molecules is carried out by three DNA-dependent RNA polymerases in eukaryotic cells. RNA polymerase I (Pol I) synthesizes the rRNA precursor, RNA polymerase II (Pol II) synthesizes messenger RNAs and various regulatory non-coding RNAs, and RNA polymerase III (Pol III) synthesizes small RNAs, including all tRNAs, 5SrRNAs, 7SLRNAs, U6 small nuclear RNAs, as well as mobile genetic elements referred to as SINEs. A key general transcription factor in Pol II machinery is TATA-binding protein (TBP) that recognizes the TATA box in the basal promoters of RNA genes. We have previously shown that its binding position in the vicinity of -28 bp from the transcription start (TSS) is determined by local structural properties of DNA at this site. TBP is the component of Pol I and Pol II transcription complexes also. The aim of this work was to find analogical binding site of TBP in the region upstream tRNA genes in eukaryotic genomes.