Accepted_test

Modeling spatial organization of lampbrush chromosomes
by Lagunov T. | Konstantinov V. | Gridina M. | Nurislamov A. | Popov A. | Kulikova T. | Krasikova A. | Fishman V. | ICG SB RAS, NSU | ICG SB RAS, NSU | ICG SB RAS | ICG SB RAS, NSU | ICG SB RAS, NSU | St. Petersburg State University | St. Petersburg State University | ICG SB RAS, NSU
Abstract ID: 518
Event: BGRS-abstracts
Sections: [Sym 1] Section “Fundamental and applied 3D genomics”

Lampbrush chromosomes represent a distinctive spatial arrangement of chromatin formed in the oocytes of most animals and oviparous mammals (but not other mammals). This structure is characterized by the large nucleus size, elongated chromosome shapes, and a very high levels of transcription. The identification of essential mechanisms underlining formation of such a structure, followed by the modeling of spatial organization, is an important task for fundamental biology of chromatin.

Based on the results of our work, we consider that the formation of lampbrush chromosomes occurs mainly due to very active transcription. A large amount of RNA matrix increases the persistent length of active transcription sites and results in the formation loops. The length of loops is further increased due to the nucleosomes removal. Most of the contact patterns on the Hi-C map were explained by the interaction of the SMC complex with active polymerases.