Accepted_test

Screening for crystallization conditions of ruminant’s chymosins
by Mironova Еkaterina | Belenkaya Svetlana | Shcherbakov Dmitry | Borshchevskiy Valentin | Arkhipov Sergey | Kolybalov Dmitry | Volosnikova Ekaterina | SRF \"SKIF\" | Center of Virology and Biotechnology «Vector» | The State Research Center of Virology and Biotechnology «Vector» | Moscow Institute of Physics and Technology | SRF \"SKIF\" | SRF \"SKIF\" | The State Researc Center of Virology and Biotechnology «Vector»\"
Abstract ID: 529
Event: BGRS-abstracts
Sections: [Sym 3] Section “Structural biology of proteins nucleic acids and membranes”

Chymosin, a peptidase protein widely used in the food industry, exhibits varying specificity to cow's milk kappa casein across different ruminant species. This study aimed to identify crystallization conditions for chymosins from Altai maral, elk, argali, and reindeer for X-ray diffraction analysis to elucidate their mechanisms of action and substrate specificity. Crystallization was conducted using the sitting-drop method and commercially available screening kits, with X-ray diffraction analysis performed on the maral chymosin. Successful crystallization conditions were identified for maral, elk, and argali chymosins, with the crystal structure of maral chymosin being deciphered and deposited. However, further research is needed to determine the crystallization conditions for reindeer chymosin. This study was supported by a budget project of the Ministry of Science and Higher Education of the Russian Federation for Synchrotron radiation facility SKIF, Boreskov Institute of Catalysis (FWUR-2024-0042)