Accepted_test

Secreted In Xylem (SIX) genes that was predicted  by homology search across the F. oxysporum f.sp. lini genomes. These genes usually divided into 14 distinct protein families. The repertoire of SIX genes in Folini genomes is quite conserved and consists of five families: SIX1, SIX7,  SIX10, SIX12 and SIX13, with notable exception for two low-virulent strains F365 and F482. Each of SIX1, SIX10 and SIX13 gene families usually present in one copy per genome, while SIX7 and SIX12 genes have paralogs and extra-copies in the individual genomes. In contrast to bulk of effectors that mostly placed in conservative genome part, SIX genes were exclusively found on variable chromosomes 12, 13 or 15. Differential expression analysis of SIX genes revealed that none of them was expressed in liquid culture mycelium, but during infestation on flax all of detected SIX genes in MI39 genome gained their expression.