Accepted_test

Such indicators of apoptotic activity as the number of cells with sickle-shaped nuclei, cells with pyknosis of the nucleus, as well as the ratio of the number of such cells to the number of living cells did not reveal differences between cells under the influence of immortelle extract and control. However, the number of cells that disintegrated into apoptotic bodies under the influence of immortelle extract at concentrations from 0.9 to 3.6 mg/mg after 24 hours was slightly higher than in the control, which indicates the presence of weak apoptotic activity of the extract as a whole. Weak apoptotic activity was also detected after 48 hours on the second day: at an extract concentration of 0.9 mg/ml, after 48 hours there is an increase in cells that disintegrated into apoptotic bodies, compared with 24 hours. Thus, the extract of immortelle sandy has a weak apoptotic effect on the culture of human kidney cancer cells A498.