Accepted_test

Impact of Lactobacilli and Bifidobacteria on Mouse Gut Mi-crobiota: Comparative Analysis of Sequencing Methods Using Illumina and Oxford Nanopore Technologies Platforms
by Klimina K. | Zoruk P. | Strokach A. | Veselovsky V. | Babenko V. | Koldman S. | Koldman V. | Odorskaya M. | Danilenko V. | Selezneva O. | Zakharievich N. | Larin A. | Morozov M. | Olekhnovich E. | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Vavilov Institute of General Genetics, Russian Academy of Sciences | Vavilov Institute of General Genetics, Russian Academy of Sciences | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine | Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
Abstract ID: 566
Event: BGRS-abstracts
Sections: [Sym 8] Section “Biotechnology through the lens of the microbiome”

Understanding the composition of the gut microbiota and its dynamics under various conditions is essential for advancing biological research. The advent of next-generation sequencing technologies, such as Illumina and Oxford Nanopore Technologies (ONT), has revolutionized studies of the microbiota by providing detailed insights into microbial communities. This study assesses the impact of the strains Lacticaseibacillus rhamnosus K32 and Bifidobacterium adolescentis 150 on mouse gut microbiota, utilizing both Illumina and ONT platforms to compare the effectiveness of 16S rRNA sequencing and whole-genome sequencing (WGS) in microbial profiling.

The introduction of these probiotic strains significantly altered the gut microbiota's composition, promoting the growth of beneficial bacteria and reducing the presence of pathogens. The analysis of sequencing data from 16S rRNA and WGS across both Illumina and ONT platforms shows that employing various sequencing techniques can enhance the representation of the intestinal microbiota's composition. Notably, the quality of the original DNA does not significantly influence the results.

This study underscores the importance of selecting appropriate sequencing strategies to thoroughly investigate the microbiome. It highlights the potential of probiotics to modulate gut microbiota, paving the way for new treatments related to gut health. Additionally, the results emphasize the complementary strengths of the Illumina and ONT platforms in microbiome studies, suggesting that a combined approach may maximize the benefits of both technologies for deeper microbiological insights.