Accepted_test

Detection of single nucleotide polymorphisms (SNPs) in nucleic acids is an urgent task of modern biomedicine. One of the approaches allowing their detection is the creation of highly specific fluorescent oligonucleotide probes.
Native oligonucleotides are colorless and small, making them difficult to visualize in cells. One of the most important breakthroughs of the 1960’s was that it became possible to modify native oligonucleotides , due to which they acquired new functions, for example, fluorescence. Such nucleotides are called fluorophores;they help detect changes in the environment and composition of oligonucleotides by changing their own fluorescence intensity.
Most studies in the field of development and research of fluorophores have only focused on creating simple and cheap to synthesize molecules. But the results of previous studies have proved inconclusive because they strongly disturb the DNA structure or have insufficient fluorescence intensity.
The main goal of my research is to test the physicochemical and structural properties of DNA duplexes containing a previously unexplored fluorophore: 8-oxo-N6-((3-methyl)-2-benzothiazolone)- 2′-deoxyadenosine (X). The main purpose of my study is to gain an understanding if this fluorophore can be used to search for SNPs.