Accepted_test
Formation of a pool of different-sized virus-specific short RNAs in Solanum tuberosum.
Our study presents findings from high-throughput sequencing (HTS) analysis of small RNAs, highlighting RNAi triggered by PVY infection and the application of dsRNA matching a segment of the PVY genome. Surprisingly, while PVY infection typically leads to the production of 21- and 22-nt mRNA species, the introduction of external PVY dsRNA resulted in the creation of an unconventional range of mRNAs spanning approximately 18–30 nt, suggesting an unforeseen pathway for small RNA generation.