Accepted_test

Identifying the transcriptional regulation targets of the insulator protein BEAF32 and investigating its role in maintaining piRNA clusters in the Drosophila melanogaster germline

by Olesya Sokolova | Koltzov Institute of Developmental Biology of RAS, Moscow, Russia

Abstract ID: 616

Event: BGRS-abstracts

Sections: [Sym 11] Section “Transcription, splicing, translation”

Insulator complexes, or boundary elements of the genome, play a crucial role in the organization of chromatin structure and the regulation of gene expression. The insulator protein BEAF32 (Boundary Element-Associated Factor 32 kDa) is one of the key insulator proteins in Drosophila. This study conducted a whole-genome analysis of gene expression in the BEAF32_KO mutant line compared to the control. Thus, the effect of the beaf32 mutation on the expression of the studied genes has a tissue-specific character.

We focused on studying the role of BEAF32 in maintaining piRNA clusters. The flamenco locus is a single-strand piRNA cluster that produces short piRNAs in the female germline. Analysis of ChIP-seq data using antibodies targeting BEAF32, Chriz, and DREF revealed that in the regulatory region of the flamenco locus the insulator protein BEAF32 is present. Its partners, Chriz and DREF, are also detected in this region.

The study of BEAF32's role in germinal tissues for the first time demonstrates the importance of insulator proteins in regulating the transcription of tissue-specific genes and the functioning of piRNA clusters, as well as the piRNAs production.

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