Accepted_test

Поиск и описание разнообразия, эволюции систем рестрикции-модификации в гипертермофильных бактериях и археях.

by Анастасия Ивановна Попова | Русинов Иван Сергеевич | Алексеевский Андрей Владимирович | НИИ Физико-химической биологии имени А. Н. Белозерского МГУ | НИИ Физико-химической биологии имени А. Н. Белозерского МГУ | НИИ Физико-химической биологии имени А. Н. Белозерского МГУ

Abstract ID: 626

Event: BGRS-abstracts

Sections: [Sym 8] Modeling and computer analysis of microbiological systems and processes

Restriction-modification (RM) systems are prokaryotic systems that include at least two enzymes: restriction endonuclease (ER) and DNA methyltransferase (MTase). ERs are actively used in genetic engineering and biotechnology. Some experiments require thermostable ERs. The purpose of the work is to study R-M systems in 7 genera of hyperthermophiles. In the genus Thermococcus the most common systems were the first type, in the genus Thermotoga the most common systems were the composition R|M, in the genus Thermus the most common systems were the compositions R|M and RM, Thermocrinis, Sulfolobus, Pyrobaculum the most common systems were the composition R|M. Atypical systems were most often found in strains of the species Thermus thermophilus and strains of uncertain species in the genus Thermococcus.