Accepted_test

Identification of the RNA-binding region of the cold shock protein CspA from Mycobacterium tuberculosis using molecular docking and molecular dynamics methods

by Pankratova Polina | Institute of Protein Research, Russian Academy of Sciences, Pushchino

Abstract ID: 631

Event: BGRS-abstracts

Sections: [Sym 3] Section “Pharmacology cheminformatics and chemical biology”

Small non-coding RNAs (sRNAs) are post-transcriptional regulators of gene expression at the translational level and can determine the stability of mRNAs in bacterial cells. They allow bacteria to rapidly adapt to changes in their environment and various stress conditions. The interaction between sRNAs and their targets is often facilitated by specialized RNA-binding proteins, known as RNA chaperones. In M. tuberculosis, the classical RNA chaperone Hfq and ProQ have not been identified. Proteins with the Cold Shock Domain (Csd) in M. tuberculosis may serve as candidates for RNA chaperones, as they perform a wide range of biological functions in the cells of various organisms. To determine the possible interactions of CspA an expert model and modelling of the reciprocal binding sites with RNA was carried out. This made it possible to identificate the surface for the interaction of proteins and localize binding sites in them.