Accepted_test

Knockout of Hsp70 genes causes changes in the transcriptome of leg skeletal muscles and locomotion speed in D. melanogaster comparable to age-related changes

by Kurochkina N.S. | Makhnovskii P.A. | Kukushkina I.V. | Popov D.V. | Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia | Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia | Moscow State University | Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia

Abstract ID: 639

Event: BGRS-abstracts

Sections: Section “Genetics and systems biology of aging”

Aging is associated with complex changes, including a decrease in physical activity, mass and functional capabilities (insulin sensitivity, performance and muscle strength) of skeletal muscles. These changes are based on various molecular and cellular processes, among which the disturbance of proteostasis plays an important role. Heat shock proteins 70 (HSP70) play a key role in the regulation of proteolysis. The association of Hsp70 (Hspa) genes expression in skeletal muscle with primary aging has recently been discovered [1], but their role in age-related changes in skeletal muscle functions is not clear [2]. The aim of the work was to study the effect of knockout of Hsp70 genes on transcriptome of leg skeletal muscle and their performance and compare these changes with age–related changes in the control group (without knockout).

Knockout of six genes of the Hsp70 family causes pronounced changes in the transcriptome of leg skeletal muscles and performance (locomotion speed) in young (7-day-old) flies. A third of these transcriptomic changes overlapped with age-related changes in the control line (47 days vs. 7 days), which partially explains the decrease in locomotion speed in young Hsp70^–flies relatively young w¹¹¹⁸flies.