Accepted_test

EFFECTS OF AGE AND LONG-TERM DISUSE ON PROTEOME OF HUMAN SKELETAL MUSCLE
by Orlova M.A. | Zgoda V.G. | Vavilov N.E. | Lednev E.M. | Zhedyaev R.Yu. | Vepkhvadze T.F. | Shpakov A.V. | Efimenko A.Yu. | Popov D.V. | Institute of Biomedical Problems, RAS, Moscow, Russia | Institute of Biomedical Chemistry, Moscow, Russia | Institute of Biomedical Chemistry, Moscow, Russia | Institute of Biomedical Problems, RAS, Moscow, Russia | Institute of Biomedical Problems, RAS, Moscow, Russia | Institute of Biomedical Problems, RAS, Moscow, Russia | Institute of Biomedical Problems, RAS, Moscow, Russia | Lomonosov Moscow State University, Moscow, Russia | Institute of Biomedical Problems, RAS, Moscow, Russia
Abstract ID: 641
Event: BGRS-abstracts
Sections: Section “Genetics and systems biology of aging”

Skeletal muscle tissue undergoes pronounced changes with aging, in particular, the amount of mitochondria and muscle mass decreases, which leads to a decline in strength, endurance, fat and carbohydrate oxidation rate, and insulin sensitivity. Chronically reduced physical activity is one of the key factors inducing these changes. The aim of this work is to investigate the effect of chronic decrease in physical activity (disuse) on the human skeletal muscle proteomic profile and to compare these changes with those induced by age.

In order to investigate the effects of ageing (the combined effects of 'normal' ageing and pathological processes), older adults (72[69-77] years, n=37) with primary knee/hip osteoarthritis, a model of aging with chronic disuse and inflammation, were compared with young healthy adults (35[28-38] years, n=15). The effects of chronic long-term disuse were studied by comparing young adults with primary knee/hip osteoarthritis (39[37-42] years; n=8) with young healthy individuals. Biopsy samples from m. vastus lateralis were taken from all volunteers for proteomic profile studies.

A total of 1900 proteins that were detected in all samples were predominantly cytoskeletal, sarcomeric and mitochondrial proteins, as well as regulators of carbohydrate and fat metabolism. In elderly patients, we observe large-scale changes in the proteome, associated mainly with a decrease in mitochondrial protein expression and an increase in inflammatory protein expression. These changes are induced by different factors: long-term disuse leads mainly to an increase in the expression of inflammatory proteins, whereas the decrease in mitochondrial proteins is mainly related to age.