Accepted_test

Distribution of epidermal proliferating cells in the model of human skin xenograft

by Cherkashina O. | Tsitrina A. | Kosykh A. | Vorotelyak E. | Kalabusheva E. | Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334 Moscow, Russia | Ilse Katz Institute of Nanoscale Science, Beer Sheva, Israel | Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian Na-tional Research Medical University, 117997 Moscow, Russia | Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334 Moscow, Russia | Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334 Moscow, Russia

Abstract ID: 659

Event: BGRS-abstracts

Sections: [Sym 9] Section “Gene expression and human diseases”

The precise pattern of epidermal proliferation is controversial. The study aimed to investigate the dynamics of cell proliferation in human skin regeneration using a xenotransplantation model. We focused on understanding the patterns of cellular division crucial for skin regeneration and normal skin function. To this end, cell proliferation was analysed at different time points post-transplantation using BrdU labeling and Ki67 staining. Cluster analysis of dividing epithelial cells provided insights into cell distribution patterns. Ki67 and BrdU markers revealed active cell proliferation, with Ki67+BrdU+ cells predominantly located in rete ridges, indicating higher proliferation rates in these regions. This research contributes valuable insights into the mechanisms of skin regeneration and the role of specific epidermal domains in cell proliferation.