Accepted_test

This study investigates the impact of Single Prolonged Stress (SPS) protocol on stress hormone receptor expression and neuroinflammation in the prefrontal cortex and hippocampus of male mice. SPS, a widely used rodent model for post-traumatic stress disorder (PTSD), was administered to one group, while another served as the control. Behavioral tests, gene expression analysis, and immunohistochemistry were conducted to assess the effects. Results reveal decreased research activity and increased anxiety levels in stressed mice, along with altered expression of stress hormone receptors and heightened neuroinflammation in the hippocampus. These findings underscore the complex neurobiological effects of stress on brain regions associated with fear processing and memory consolidation.