Accepted_test

Association of SNP in the KAT6B gene with live weight of heifers of the Yaroslavl cattle breed
by Igoshin A.V. | Yudin N.S. | Ilina A.V. | Larkin D.M. | Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia | Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia | Federal Williams Research Center for Forage Production and Agroecology, Scientific Research Institute of Livestock Breeding and Forage Production, Yaroslavl Region, Russia | Royal Veterinary College, University of London, London, United Kingdom
Abstract ID: 707
Event: BGRS-abstracts
Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

Previously, using the whole genome genotyping, we showed that the Yaroslavl breed has unique genetics compared to other Russian and foreign cattle breeds, while foreign breeds had an insignificant impact on the gene pool of Yaroslavl cattle. Analysis of whole genome sequencing data of the Yaroslavl cows and data from the "1000 Bull Genomes" project made it possible to identify a unique haplotype on chromosome 28, which is found almost exclusively in Yaroslavl cattle breed. This haplotype contains the KAT6B gene and appears to be negatively associated with withers height, heart girth and live weight at different ages. So probably, it has undergone purifying selection in other cattle breeds.

DNA fragment containing the missense variant G>A (p.Val105Met) within the KAT6B gene was amplified by PCR. The amplicons were cut with HpySE526 I restriction enzyme. The association between phenotypes obtained from individual heifer record cards and genotypes was estimated using linear regression (‘lm’ R function).

We genotyped an additional 112 heifers to study the negative association of the Val105Met mutation in the KAT6B gene with live weight in Yaroslavl cows. The association we found earlier was confirmed in a pooled sample of 142 animals. Thus, the Yaroslavl cattle-specific Val105Met variant in the KAT6B gene is negatively associated with live weight of heifers at different ages. A further study exploring the relation of this mutation to other economically important phenotypes will elucidate its potential for use in marker-assisted selection.