Accepted_test

Creation of effective strains-producers is impossible without a solid base in the form of a collection of microorganisms with biotechnological properties. For this purpose, in addition to purposeful search, collection and isolation of microorganisms, it is necessary to study them comprehensively using classical and modern tools, methods and approaches, in particular proteomics, transcriptomics, metabolomics and bioinformatics, allowing to analyse the obtained data in order to search for and create new effective strains-producers.

The collection of microorganisms for biotechnological purposes of FRC ICG SB RAS has been established and is constantly replenished with new cultures. Currently, the collection includes more than 3000 cultures, among which there are representatives of not only different phyla, but also domains. Genes of target enzymes necessary for modern biotechnological processes, were found in the genomes of the strains. On this basis, the target genes were cloned into the genome of yeast Komagataella phaffii T07 and effective strains-producers and preparations of enzymes amylase, mannanase, xylanase, protease, cellulase, etc. were obtained.

The collection of microorganisms for biotechnological purposes at the FSC ISG SB RAS implements an integrated approach, which allowed to form a solid base for genetic, molecular-biological and biotechnological research and allows to obtain effective strains-producers and enzyme preparations.

The study is supported by the Ministry of Science and Higher Education of the Russian Federation (№ FWNR-2022-0022) and Kurchatov Genomic Center, Federal Research Center ICG SB RAS (№ 075-15-2019-1662).