Accepted_test

The microbiota is characterized by a wide range of secretory activities. Qualitative modifications of the microbiome occur during inflammatory diseases, which is largely reflected in the composition of the volatile metabolome. Functional metabolomics contributes to the assessment of the dynamic state of the microbiota in health and disease, using data from metagenomic and metabolomic analysis to form a comprehensive description of the state of the microbiota. If standard sequencing methods are used to assess the meta-genome, then various methodological approaches are considered to study the volatile spectrum of the metabolome. In this research, the GC-MS with a headspace extraction method was used to assess the volatile spectrum of microbiota metabolites. To assess the dynamic state of the microbiota, experiments were conducted on the formation of DSS-induced colitis in animals (rat/mice), followed by inflammation treatment by Bacteroides fragilis (JIM) vesicles, which are known to have a immunomodulatory properties.