Accepted_test

Striatum Transcriptome Profile Shift in Repeated Aggression Mouse Model quickly recovered to normal except for 12 circadian clock genes after Fighting Deprivation
by Vladimir Babenko | ICG SB RAS, Novosibitsk, Russia
Abstract ID: 798
Event: BGRS-abstracts
Sections: [Sym 1] Section “Genome-wide transcriptomics (differential gene expression)”

Vladimir Babenko  1 , Olga Redina  1 , Dmitry Smagin  1 , Irina Kovalenko  1 , Anna Galyamina  1 , Roman Babenko  1 , Natalia Kudryavtseva  1   2

 

Affiliations

1Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.

2 Pavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint Petersburg, Russia.

Abstract

Aggressive species were subjected to repeated social conflict encounters (fights) with regular positive (winners) experience in the course of 20 consecutive days (A20 group). This led to a profoundly shifted transcriptome expression profile relative to the control group, outlined by more than 1000 differentially expressed genes (DEGs). Fourteen days of a consecutive fighting deprivation period (AD group) featured the basic restoration of the normal (control) transcriptome expression profile yielding only 62 DEGs against the control. Notably, we observed a network of 12 coordinated DEG Transcription Factor (TF) activators from 62 DEGs in total that were distinctly altered in AD compared to control group, underlining the distinct transcription programs featuring AD group, partly retained from the aggressive encounters and not restored to normal in 14 days. We found circadian clock TFs among them, reported previously as a withdrawal effect factor.

Keywords: addiction; behavior; cAMP cascade; chronic social conflict; dopamine; dorsal striatum; endogenous opioids; nucleus accumbens; transcriptome.