Accepted_test

Phylostratigraphic analysis of gene networks of cell cycle in animals.

by Turnaev Igor | Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Kurchatov Genomic Center of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia

Abstract ID: 88

Event: BGRS-abstracts

Sections: [Sym 7] Section “Genomics, genetics and systems biology of animals”

We analyzed PAI (Age index) in cell cycle (CC) genes. The most ancient were 12 genes (9%) originated in cellular organisms. The origin of 59% of the genes of the CC network corresponds to the appearance of unicellular eukaryotes. Another 27% of genes appeared with multicellular eukaryotes, 5 genes (4%) appeared in evolution in vertebrates.

Genes of the E2F family of transcription factors E2F2-6 appeared in evolution in unicellular eukaryotes and only E2F1 appeared in multicellular eukaryotes. RB family genes RB1 and RBL1 appeared in evolution with the appearance of unicellular eukaryotes, but the RBL2 gene - at the appearance of multicellular eukaryotes. Genes of the cyclin family mainly appeared in unicellular eukaryotes and only cyclins D2, E1 and E2 appeared in multicellular eukaryotes. Almost all genes of the CDK family originated in unicellular eukaryotes. Interestingly, genes of CDIs appear somewhat later in evolution.

All young СC genes (that occurred in chordates, vertebrates, and bony vertebrates) are characterized by a low level of DI (divergence index) gene (<0.3). This indicates that these genes are under high selective pressure.

Analysis of the ages (PAI index) of the genes of the CC network showed that genes of the major CC families E2F, RB, CDK, and cyclins appeared in evolution together with the formation of unicellular or multicellular eukaryotes. Whereas CDI family genes appeared somewhat later mainly in multicellular eukaryotes and in vertebrates. This may be due to the need for tighter control of CC in multicellular eukaryotes and vertebrates.