Laser 3D-modeling in research of molecular features of skin lymphatic vessels in the patients with urticaria pigmentosa

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Svetlana Viktorovna Michurina¹, Svechnikova Natalia Nikolaevna², Andrey Yurievich Letyagin³, Irina Yurievna Ishchenko⁴, Sergey Alekseevich Arkhipov⁵, Solovyova Anastasia Olegovna⁶, Konenkov Vladimir Iosifovich^7
1Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru
²Laboratory of clinical immunogenetics SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, n.svechnikova@ngs.ru
³Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, letyagin-andrey@yandex.ru
⁴Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru
⁵Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, arhipowsergei@yandex.ru
⁶Laboratory of pharmacological active compounds SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, solovevaao@gmail.com
⁷Laboratory of clinical immunogenetics SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, vikonenkov@gmail.com

Congestion in the blood and lymphatic vessels is detected in the papillary and reticular layers in the skin dermis of patients with pigment urticarial. This promotes the development of microcirculatory disorders and the formation of perivascular infiltrates. LYVE-1 positive staining was detected not only in the endothelium of the lymphatic vessels (both in the skin papillary layer and in the deeper dermis layers), but also in the prickle cells of the stratum spinosum and (less pronounced) in the cells of the stratum basal in the epidermis. Our 3D modeling of the LYVE-1 marker expression in skin epidermis suggests that mainly the prickle cells of the epithelium stratum spinosum and to a lesser extent the cells of the epithelium basal layer in patients with cutaneous mastocytosis participate in the lymphatic transport of hyaluronan and the spaces between these cells can be considered as prelimfatiks of the skin epidermis.