Human Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

Poster (download) Alexander Lykov1, Lubov Rachkovskaya2, Olga Poveshchenko3, Maria Surovtseva4, Irina Kim5, Edmund Rachkovsky6, Alena Philippova71Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, aplykov2@mail.ru2Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru3Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, poveschenkoov@yandex.ru4Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, mfelde@ngs.ru5Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, kii5@yandex.ru6Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru7Municipal autonomous educational institution Education center Gornostay, alena.philippova2003@yandex.ru Fibroblasts and mesenchymal stem cells are involved in damaged skin repair. Functional activity of the fibroblasts mesenchymal stem cells depends from environment, including antimicrobial preparations. Silver salts are one of the antimicrobial substances. Lithium salts exhibit not only a stabilizing effect on human mental responses, but also antitumor and autophagic effects. The “Bodyguard” hygienic composition contains both silver and lithium. The aim of the study was to study the effect of bound silver and lithium ions in the \”Bodyguard\” composition and in salt of silver nitrate and lithium of citric acid on human dermal fibroblasts (DFs) and bone-marrow mesenchymal stem cells (MSCs) functions. It has been shown that DFs in the presence of \”Bodyguard\” reduces proliferative activity and significantly increases care in apoptosis. At the same time, silver and lithium ions alone or in combination do not affect proliferation, enhance migration and reduce DFs apoptosis. It has been shown that MSCs proliferation increased under “Bodyguard” condition. Whereas, silver ions and lithium ions not have any effect on proliferation of MSCs, but influenced on myeloperoxidase activity, nitric oxide production, migration, and apoptosis. The more pronounced negative action of the “Bodyguard” is most likely due not to silver and lithium ions, but to the very structure of the carrier to which these ions are immobilized.

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The lithium effects on morphology and apoptosis in hepatocellular carcinoma cells

Poster (download) Iuliia Taskaeva1, Izabella Gogaeva2, Natalia Obanina3, Viktoriia Makarova4, Nataliya Bgatova51Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Laboratory of boron-neutron capture therapy, Department of Physics, Novosibirsk State University, inabrite@yandex.ru2Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Department of Natural Sciences, Novosibirsk State University, i.gogaeva@g.nsu.ru3Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Section of cytology and genetics, Department of Natural Sciences, Novosibirsk State University, n.obanina@g.nsu.ru4Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, shedina_vika@mail.ru5Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, n_bgatova@ngs.ru Hepatocellular carcinoma (HCC) is characterized dysregulation of cell death mechanisms, and the imbalance of pro- and anti-apoptotic signals. The development of HCC is accompanied by genetic mutations in the signaling pathways involved in the cell proliferation, growth and death. The molecular changes in apoptosis signaling in HCC determine the requirement for targeted chemotherapy to increasing apoptosis in HCC cells. The aim of this study was to assess the ability of lithium to influence on the hepatocellular carcinoma-29 (HCC-29) cells apoptosis in vivo. Light and transmission electron microscopy, and immunofluorescence staining were used to evaluate of apoptosis development in HCC-29 cells after administration of 20 mM lithium carbonate. It was revealed that lithium extremely increased the pro-apoptotic proteins Bad and caspase-3 expression, and decreased the anti-apoptotic protein Bcl-2 expression. These results indicate that lithium carbonate induces apoptosis pathways in HCC-29 cells. Lithium administration can enhance pro-apoptotic chemotherapeutic drugs potential and overcome the resistance of tumor cells to apoptosis in HCC.

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The effect of lithium carbonate on angiogenesis of hepatocellular carcinoma-29

Viktoriia Makarova1, Nataliya Bgatova2, Iuliia Taskaeva31Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, shedina_vika@mail.ru2Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, n_bgatova@ngs.ru3Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, inabrite@yandex.ru Гепатоцеллюлярная карцинома является одной из самых агрессивных опухолей человека с высокой распространенностью и смертностью. В то же время существует проблема устойчивости ГЦК к стандартной терапии. Поиск лекарств, блокирующих рост и метастазирование опухоли, является актуальным. Имеются данные о противоопухолевых свойствах карбоната лития. Целью нашего исследования было определить влияние лития на развитие и структуру кровеносных сосудов экспериментальной гепатоцеллюлярной карциномы-29 (G-29). Исследование было выполнено на самцах мышей CBA. Клетки гепатокарциномы-29 использовали для индукции опухолевого процесса. Изучали экспрессию эндотелиального маркера кровеносных сосудов (CD-31) и ультраструктурную организацию опухолевых сосудов эндотелиальных клеток. Было показано, что карбонат лития уменьшает объемную плотность кровеносных сосудов G-29. Ультраструктурный анализ опухолевых сосудов выявил нетипичный характер их строения. Стенки сосудов были выстланы как эндотелиальными, так и опухолевыми клетками.

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