The expression of Bcl-2 family proteins in liver cells of 57Bl/6 mice under conditions of functional pinealectomy

Poster (download) Svetlana Viktorovna Michurina1, Irina Yurievna Ishchenko2, Sergey Alekseevich Arkhipov3, Andrey Yurievich Letyagin4, Maxim Aleksandrovich Korolev5, Evgenii Leonidovich Zavjalov6 1Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru 2Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru 3Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, arhipowsergei@yandex.ru 4Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, letyagin-andrey@yandex.ru 5Laboratory of connective tissue pathology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, kormax@bk.ru 6Center of genetis resources of laboratory animals ICG SB RAS Novosibirsk, Russia, zavjalov@bionet.nsc.ru The expression of antiapoptotic Bcl-2 protein and the proapoptotic Bad protein in liver cells of C57BL/6 mice males kept 14 days under 24-hour lighting (24hL) (photoperiod light/dark 24/0 hours) was studied. Control animals kept under standard lighting conditions (photoperiod light/dark 14/10 hours). The immunohistochemical analysis (indirect avidin-biotin-streptavidin method) and morphometric assessment were used. We found in the liver cells of mice under round-the-clock lighting the increase in the area of Bcl-2 protein expression without changing the area of Bad protein expression, that led to a decrease in the ratio of Bcl-2/Bad. This result indicated weakening of the antiapoptotic protection of organ cells and creates conditions for activation of the “mitochondrial branch” of apoptosis in animal liver cells with functional pinealectomy.

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LYVE-1 expression in liver cells of mice with functional pinealectomy

Poster (download) Irina Yurievna Ishchenko1, Svetlana Viktorovna Michurina2, Sergey Alekseevich Arkhipov3, Andrey Yurievich Letyagin4, Maxim Aleksandrovich Korolev5, Evgenii Leonidovich Zavjalov61Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru2Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru3Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, arhipowsergei@yandex.ru4Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, letyagin-andrey@yandex.ru5Laboratory of connective tissue pathology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, kormax@bk.ru6Center of genetis resources of laboratory animals ICG SB RAS Novosibirsk, Russia, zavjalov@bionet.nsc.ru The expression of the lymphatic vessel endothelial receptor-1 hyaluronan-1 (LYVE-1) was studied in the liver cells of male C57BL / 6 mice, which were kept for 14 days under 24-hour illumination (mice with functional pinealectomy). Used immunohistochemical analysis (indirect avidin-biotin-ABC-peroxidase method) and morphometric evaluation. A 2-fold decrease in the LIVE-1 expression area was detected against a 2% increase in the optical density of LIVE-1 staining in mouse liver cells after chronic continuous illumination. Weak expression of LYVE-1 on the membranes of endothelial cells of the sinusoids of the liver may indicate a violation of the functioning of the fields of fenestration of these cells. This can lead to a decrease in the endocytotic activity of the latter, difficulty in the exchange of hematotissue tissues, deterioration of lymphatic drainage and the development of tissue hypoxia in the liver of mice with functional pinealectomy.

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The development of compensatory processes in the liver and kidney in conditions of distant tumor growth

Poster (download) Nataliya Bgatova1, Asel Rakhmetova2, Saule Bakhbaeva3, Viktoriia Makarova4, Iuliia Taskaeva51Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences,Novosibirsk, Russia, n_bgatova@ngs.ru2Pavlodar State University; Pavladar, Kazakhsta, asel-rakhmetova@mail.ru3Pavlodar State University; Pavladar, Kazakhstan, saule0577@mail.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, shedina_vika@mail.ru5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, inabrite@yandex.ru Development of malignant tumor accompanied endogenous intoxication of an organism having a mixed nature, in particular due to dysfunction of organs detoxification and excretion damaging effect of tumor metabolism. The active process of moving toxic and biologically active substances formed during tumor progression from the primary focus by blood and lymph flow leads to damage of distant organ. The aim of this work was to identify structural changes in the liver and kidney under the conditions of modeling distant tumor growth. For modelingВ  tumor growth, hepatocarcinoma-29 (G-29) cells were used. G-29 cells were injected into the muscle of the right thigh of CBA mice [1]. The material for research was collected after 3, 7, 13, and 30 days of the experiment. The structure of the liver and kidney was studied by the method of light and electron microscopy. Both destructive and compensatory changes in the liver and kidney were revealed in conditions of distant tumor growth. In the liver, a decrease a size of hepatocytes and concentration of organelles, as well as development of autophagy, as a mechanism for maintaining intracellular homeostasis, were noted. In the kidney at the early stages of the development of the tumor growth, structural signs of filtration barrier disorde were shown. Subsequent development of compensatory hypertrophy of podocytes and glomerular capillary endothelium, as well as autophagy in the epithelium of the proximal nephron were noted.

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