The expression of Bcl-2 family proteins in liver cells of 57Bl/6 mice under conditions of functional pinealectomy

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Svetlana Viktorovna Michurina¹, Irina Yurievna Ishchenko², Sergey Alekseevich Arkhipov³, Andrey Yurievich Letyagin⁴, Maxim Aleksandrovich Korolev⁵, Evgenii Leonidovich Zavjalov^6
1Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru
²Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru
³Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, arhipowsergei@yandex.ru
⁴Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, letyagin-andrey@yandex.ru
⁵Laboratory of connective tissue pathology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, kormax@bk.ru
⁶Center of genetis resources of laboratory animals ICG SB RAS Novosibirsk, Russia, zavjalov@bionet.nsc.ru

The expression of antiapoptotic Bcl-2 protein and the proapoptotic Bad protein in liver cells of C57BL/6 mice males kept 14 days under 24-hour lighting (24hL) (photoperiod light/dark 24/0 hours) was studied. Control animals kept under standard lighting conditions (photoperiod light/dark 14/10 hours). The immunohistochemical analysis (indirect avidin-biotin-streptavidin method) and morphometric assessment were used. We found in the liver cells of mice under round-the-clock lighting the increase in the area of Bcl-2 protein expression without changing the area of Bad protein expression, that led to a decrease in the ratio of Bcl-2/Bad. This result indicated weakening of the antiapoptotic protection of organ cells and creates conditions for activation of the “mitochondrial branch” of apoptosis in animal liver cells with functional pinealectomy.