• June 29, 2020
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Correlation between Cytokines Lymph and Mesenteric Lymph Nodes on Breast Cancer in Rat

Poster (download) Kazakov Oleg1, Poveshchenko Alexander2, Orlov Nikolai3, Kabakov Alexey4, Lykov Alexander5, Strunkin Dmitry6, Konenkov Vladimir71Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, kazakoff_oleg@mail.ru2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, poveshchenkoa200@mail.ru3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, nbo@ngs.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, kabakov_av85@mail.ru5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, aplykov2@mail.ru6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Strunkind@mail.ru7Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com The correlation analysis of the morphometry of mesenteric lymph nodes and cytokines of the chest duct lymph in chemically induced breast cancer was carried out. The study showed that in breast cancer, the activity of the local immune response in the lymph nodes is aimed at antitumor protection. In breast cancer, the area of the paracortical zone remains at the level of the intact group, the area of lymphoid nodules with germinative centers and the area of medullary substance are increased, the number of macrophages in the thymus-dependent zone and zone responsible for humoral immunity is increased. In breast cancer positive correlation were revealed: in the germinative centers and medullary substance – mitotically dividing cells with cytokine IL-5, medium lymphocytes with chemokine MIP-1О±, in the germinative centers – immunoblasts with cytokine GRO/KC, in the paracortical zone – chemokine MCP-1 with macrophages, reticular cells with IL-6 and M-CSF, in medullary substance – small lymphocytes and mature cells plasma cells (the number of which is decrease) with chemokine Gro/KC, that can be caused by their migration from lymph nodes.В 

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  • June 29, 2020
  • N/A

Relationship Cytokines Lymphs and Mesenteric Lymph Nodes Rats at Chemotherapy Breast Cancer

Poster (download) Kazakov Oleg1, Poveshchenko Alexander2, Orlov Nikolai3, Kabakov Alexey4, Lykov Alexander5, Strunkin Dmitry6, Konenkov Vladimir71Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kazakoff_oleg@mail.ru2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, poveshchenkoa200@mail.ru3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, nbo@ngs.ru4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kabakov_av85@mail.ru5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, aplykov2@mail.ru6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, Strunkind@mail.ru7Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com At correlating the morphometry of mesenteric lymph nodes and the concentration of cytokines in the lymph of the thoracic duct in breast cancer induced by intramammary administration of N-methyl-n-nitrosourea, chemotherapy according to the CMF scheme, compared with breast cancer without treatment, positive relationships were found that may indicate on increase in the immunomodulatory and antitumor action of cytokines. The correlation of interferon IFNОі with small lymphocytes (the number which increased) and macrophages in germinative centers and mitotic dividing cells in medullary substance, correlation in germinative centers of immunoblasts with MIP-1О± and increase of number small lymphocytes in the thymus dependent zone of lymph nodes, correlation in medullary substance of interleukin IL-17 with mature plasma cells (the number of which is increased), correlation of interleukin IL-18 with mature plasma cells in the medullary sinuses.

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CYTOKINES – MARKERS OF ONCOGENESIS AND THERAPY EFFICIENCY IN CHEMICALLY INDUCED BREAST CANCER

Poster (download) Alexandr Poveshchenko11Research Institute of Clinical andExperimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, poveshchenkoa200@mail.ru Breast cancer was induced by the administration of n-methyl-N-nitrosourea to Wistar rats. Some animals underwent only surgical intervention or only polychemotherapy (cyclophosphamide, methotrexate, 5-ftorouracil). In some animals, both types of therapy were combined, and in a separate group, the administration of the Panagen drug, which was a fragmented DNA molecule, was added to polychemotherapy. To study the concentration of cytokines in lymph, we used the Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad, USA). It was shown that in rats with breast cancer the content in the lymph and blood of most of the studied cytokines such as IL-1ОІ, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1О±, MIP-3О±, RANTES, TNF-О±, MCP-1 were statistically significantly higher, and IL-10 and GRO / KC, lower than in intact animals. Surgical removal of the tumor led to a significant decrease in the content of both pro-inflammatory and anti-inflammatory cytokines in the lymph. PCT led to a significant decrease in the content of IL-1ОІ, IL-4, IL-6, IL-7, MIP-1О±, MIP-3О±, RANTES in the blood serum and lymph of rats with breast cancer.

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MICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

Poster (download) Aleksey Kabakov1, Alexandr Lykov2, Oleg Kazakov3, Alexandr Poveshchenko4, Olga Poveshchenko5, Dmitriy Strunkin6, Lyudmila Gulyaeva7, Andrey Letyagin8, Vladimir Konenkov91RICEL-Branch of IC&G SB RAS, kabakov_av@mail.ru2RICEL-Branch of IC&G SB RAS, aplykov2@mail.ru3RICEL-Branch of IC&G SB RAS, kazakoff_oleg@mail.ru4RICEL-Branch of IC&G SB RAS, poveshchenkoa200@mail.ru5RICEL-Branch of IC&G SB RAS, poveschenkoov@yandex.ru6RIMBB FRC FTM, strunkind@mail.ru7RIMBB FRC FTM, gulyaeva@soramn.ru8RICEL-Branch of IC&G SB RAS, letyaginay@bionet.nsc.ru9RICEL-Branch of IC&G SB RAS, vikonenkov@gmail.com The study of concentration miRNA (21, 221, 222, and 429) in serum, lymph of the thoracic duct and breast cancer tissue, as well as the cellular composition of axillary lymph node in Wistar female rats in chemically induced breast cancer. Concentration microRNA (-221, -429) in the tissues breast cancer and lymph are correlated with a decrease in number of lymphocytes in medullary cords of axillary lymph nodes.

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Proteins and Cytokines of Blood Cells Supernatant and Histological Atypical Cells Markers in the Breast Diseases

Poster (download) Video (download) Kristina Davletova1, Albina Bernado2, Sergey Arkhipov3, Igor Zhurakovsky4, Nikolay Varaksin5, Alexander Autenshlyus161Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, christina.davletova@gmail.com2Novosibirsk State Medical University, idontbelive69@mail.ru3Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, arhipowsergei@yandex.ru4Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, murash2003@yandex.ru5AO Vector-Best, varaksin@vector-best.ru6Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, lpciip@211.ru The study examined the concentration of proteins and cytokines in the supernatants of blood cells, as well as the expression of histological markers of atypical cells in breast diseases. In addition, he studies the correlation between concentrations of proteins and cytokines and histological markers of atypical cells. It was found that the concentration of histidine-rich glycoprotein in supernatants of blood cells with a special type of invasive carcinoma is higher compared with benign breast diseases. In addition, the following statistical relationships were observed in the study: negative correlation between estrogen receptor-α concentrations and integrin-1 expression; a positive correlation between the concentrations of histidine-rich glycoprotein and expression of type II collagen; a positive correlation between e-cadherin concentrations and e-cadherin expression; and a positive correlation between the concentrations of plasminogen-1 activator inhibitor and the expression of type II collagen. The production of cytokines by blood cells is identified as an index of the influence of polyclonal activators (a combination of mitogens). The study shows the difference in the index of influence on the production of IL-1RA and TNF-α between benign breast diseases with a high and low risk of malignant transformation and between benign breast diseases with a high risk of malignant transformation and breast cancer with metastases to the lymph nodes. The index of the effect of polyclonal activators on IL-1RA production increases as the prognosis of the disease worsens. As blood samples and tumors show, patients with benign breast diseases have a negative correlation between the index of influence of polyclonal activators on IL-1RA production and expression of type II collagen. In addition, patients with breast cancer as well as breast cancer with metastases in the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal stimulation stimulated by the production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the […]

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New germline mutations in PTEN and RAD51D genes among the Buryat Mongol breast cancer patients

Polina Gervas1, Aleksey Molokov2, Nadezda Cherdyntseva31Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, pgervas@yandex.ru2Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, amolokov@inbox.ru3Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, nvch@tnimc.ru Breast cancer is the most prevalent female malignancy worldwide. In recent decades, breast cancer has been the most common malignancy in the regions of Siberia and Russian Far East, which occupy about 70% of the Russian Federation. The population is descended primarily from newcomers (Slavic ancestors) and indigenous population (Mongolian). More than 13.8 million women inhabit these regions. Currently, little information has been reported regarding the molecular factors associated with increased risk of developing hereditary breast cancer in the indigenous population (Buryats, Evenks, Altaians, Tuvinians, Khakassians). For the indigenous population, there are no standards for the treatment of inherited breast cancer. Breast cancer prevention models for indigenous population have not been developed yet. For \”slavic\” patients with a family history, the BRCA1/2 mutation testing is the standard of care. In addition, the development of new antitumour drugs has resulted in improved survival rates. More than 1000 mutations of the BRCA1 gene have been identified. Recent research is focused on the confirmation the beneficial effect of identified mutations. In our previous study we did not find \”slavic\” mutations of the BRCA1 gene in Mongoloid breast cancer patients having family history. Pathological mutations in the BRCA1 gene among indigenous people have not been identified using Sanger sequencing (RFBR grant 12-04-90830). Thus, the advances in molecular oncology for the treatment of hereditary breast cancer are not available for the indigenous population of the Siberian and Far Eastern region.

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