Proteins and Cytokines of Blood Cells Supernatant and Histological Atypical Cells Markers in the Breast Diseases

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Kristina Davletova¹, Albina Bernado², Sergey Arkhipov³, Igor Zhurakovsky⁴, Nikolay Varaksin⁵, Alexander Autenshlyus1^6
1Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, christina.davletova@gmail.com
²Novosibirsk State Medical University, idontbelive69@mail.ru
³Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, arhipowsergei@yandex.ru
⁴Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, murash2003@yandex.ru
⁵AO Vector-Best, varaksin@vector-best.ru
⁶Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, lpciip@211.ru

The study examined the concentration of proteins and cytokines in the supernatants of blood cells, as well as the expression of histological markers of atypical cells in breast diseases. In addition, he studies the correlation between concentrations of proteins and cytokines and histological markers of atypical cells. It was found that the concentration of histidine-rich glycoprotein in supernatants of blood cells with a special type of invasive carcinoma is higher compared with benign breast diseases. In addition, the following statistical relationships were observed in the study: negative correlation between estrogen receptor-α concentrations and integrin-1 expression; a positive correlation between the concentrations of histidine-rich glycoprotein and expression of type II collagen; a positive correlation between e-cadherin concentrations and e-cadherin expression; and a positive correlation between the concentrations of plasminogen-1 activator inhibitor and the expression of type II collagen. The production of cytokines by blood cells is identified as an index of the influence of polyclonal activators (a combination of mitogens). The study shows the difference in the index of influence on the production of IL-1RA and TNF-α between benign breast diseases with a high and low risk of malignant transformation and between benign breast diseases with a high risk of malignant transformation and breast cancer with metastases to the lymph nodes. The index of the effect of polyclonal activators on IL-1RA production increases as the prognosis of the disease worsens. As blood samples and tumors show, patients with benign breast diseases have a negative correlation between the index of influence of polyclonal activators on IL-1RA production and expression of type II collagen. In addition, patients with breast cancer as well as breast cancer with metastases in the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal stimulation stimulated by the production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation. and breast cancer with metastases to the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal activator-stimulated production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation. and breast cancer with metastases to the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal activator-stimulated production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation.