Darya V. Spiryova1, Alexander E. Moskalensky2, Alexei Vorob’ev3
Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile caged activation agonists. Futhermore, a mathematical model was developed that allows to detect individual calcium peaks andВ derive their dynamic parameters.