Poster (download) Shcherban A.B.1, Perfil’ev R.N2, Salina E.A.31Institute of Cytology and Genetics SB RAS, atos@bionet.nsc.ru2Novosibirsk State Agrarian University, pervf.1999@gmail.com3Institute of Cytology and Genetics SB RAS, sunday01@mail.ru Molecular markers were used to analyze the mitochondrial genome of the alloplasmic lines of wheat containing the nuclear genome of the hexaploid wheat T. aestivum against the background of the cytoplasm of tetraploid wheat T.dicoccum. Eight lines showed patterns of PCR and CAPS markers corresponding to the species T. aestivum, indicating the substitution of the mitochondrial genome of T. dicoccum during backcrossing with hexaploid wheat. In the D-N-05 line, the rps19 and orf256 gene markers corresponded to the parental species T.dicoccum. This line is promising in terms of studying the mechanisms of fertility restoration in hybrids with CMS. Also, using molecular marker, the nuclear gene Dreb-1, which is a regulator of drought tolerance, was analyzed and it was shown that  line D-41-05 had an introgression of this gene from the B genome of T. dicoccum, which may lead to the previously established increased drought tolerance of this line.

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