Circulating microRNAs potentially associated with progression of castration-resistant prostate cancer

Elena Pudova11EIMB RAS, pudova_elena@inbox.ru Metastatic castration-resistant prostate cancer (mCRPC) is characterized by a high mortality rate due to its aggressiveness. In the field of modern oncourology, monitoring the activity of a disease for patients of this category during therapy is an important clinical problem, for the solution of which informative markers are needed. Circulating microRNAs can serve as such markers, which demonstrate diagnostic and prognostic potential in various types of cancer, including prostate cancer. The aim of this study is to search for circulating miRNAs of mCRPC patients plasma during therapy that are potentially associated with the progression of the disease, as well as to study the contribution of these miRNAs to the general progression mechanism. Based on high-throughput sequencing, a bioinformatic analysis was carried out with identification of the microRNA profile potentially associated with the progression of the disease (p-value <0.05): miR-221-3p, miR-99b-3p, miR-145-5p, miR-375-3p, miR-20a-5p, miR-21-5p, miR-451a, miR-199a-5p, miR-183-5p, miR-let-7i-5p and miR-let-7f-5p.

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MicroRNA content of horse and human milk exosomes

Sergey Sedykh1, Kuleshova Anna2, Georgy Nevinsky31SB RAS ICBFM, NSU, sedyh@niboch.nsc.ru2SB RAS ICBFM, aekuleshova25@gmail.com3SB RAS ICBFM, NSU, nevinsky@niboch.nsc.ru Exosomes are 40-100 nm diameter natural vesicles, containing CD9, CD63 and CD81 tetraspanins on the surface. It was shown that milk obtained from different sources contain exosomes: human, horse, bovine, rat. Horse milk is a unique source of exosomes since is less allergenic than bovine and not prion prone. Also, horse milk can be obtained in larger amounts than human milk. According to the results, published by the other research groups, milk exosomes contain hundreds and thousands of proteins, mRNA and microRNA molecules. Our recent results show that these numbers may be significantly overestimated.В Isolation of exosomes from sediments obtained after ultracentrifugation with an additional step of gel-filtration allows decreasing the number of proteins, that co-isolate with vesicles. Many microRNAs are described in exosomes, obtained from human and bovine milk. Here we show the content of more than two dozens major microRNA molecules, isolated fromВ human and horse milk exosomes on different stages of centrifugations, ultracentrifugations, before and after gel-filtration. According to these data, several microRNAs may be used as indicators of milk exosomes\’ purity. The study was funded by the Russian Scientific Foundation (research project 187410055 to S. Sedykh) and by the Program of Fundamental Research of Government Academia (РђРђРђРђ-Рђ17-117020210023-1 to Prof. G. Nevinsky).

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