Comparative Genomic Analysis of Moderate Bacteriophages of Alfalfa Root Nodule Bacteria

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Kozlova A.P.1, Muntyan V.S.2, Afonin A.M.3, Antonova E.V.4, Muntyan A.N.5, Kabilov M.R.6, Roumiantseva M.L.7, Dzyubenko E.A.8
1All-Russian research institute for agricultural microbiology, Pushkin, Saint-Petersburg, Russia, alexsandrak95@mail.ru
2All-Russian research institute for agricultural microbiology, Pushkin, Saint-Petersburg, Russia, vucovar@yandex.ru
3All-Russian research institute for agricultural microbiology, Pushkin, Saint-Petersburg, Russia, afoninalexeym@gmail.com
4Institute of Plant and Animal Ecology, Ural Division of Russian Academy of Sciences, Ekaterinburg, Russia, selena@ipae.uran.ru
5All-Russian research institute for agricultural microbiology, Pushkin, Saint-Petersburg, Russia, allmuntyan@gmail.com
6ICBFM SB RAS, Novosibirsk, Russia, kabilov@niboch.nsc.ru
7All-Russian research institute for agricultural microbiology, Pushkin, Saint-Petersburg, Russia, mroumiantseva@yandex.ru
8Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) Ministry of science and higher education, Saint-Petersburg, Russia, elena.dzyubenko@gmail.com

The bacteriophage AP300 infecting Sinorhizobium meliloti was isolated from soil sample В from В mountainous region of Dagestan, which belongs to the Caucasus Center for Legume Variety in the frame of the grant RSFВ 17-16-01095. The AP300 genome was annotated and its similarity to bacteriophage vB MloP_Lo5R7ANS (NC_025431; Podoviridae family) infecting Mesorhizobium loti strains was revealed. In the chromosomes of 4 strains of Sinorhizobium meliloti isolated in geographically different regions from soils and nodules of alfalfa plants, intact vB MloP_Lo5R7ANS-like prophages were detected.В The 46.39-57.14% ORFsВ of AP300 and 4 prophages were homologous to ORFsВ of vB MloP_Lo5R7ANS. The 18 ORFs from mentioned above ORFs were identified in all studied sequences and they wereВ similar to those in vB MloP_Lo5R7ANS. The other 7.22-15.79% ORFsВ were homologous to other phages. ORFs encodingВ virion components: head, capsid, tail, virion, integrase were detected in soil bacteriophage AP300 and in all studied В prophages determined in rhizobia symbionts of alfalfa.

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