Computational prediction of miRNA binding sites in mRNA of colorectal cancer candidate genes

Aigul Akimniyazova^1
1SRI of Biology and Biotechnology problems, al-Farabi Kazakh National University, akimniyazova@gmail.com

The identification of causative miRNAs in colorectal cancer is unclear due to a lack of understanding of how specific miRNAs affect biological pathways and consequences of disease development. The miRNA is a class of nano-sized non-coding RNAs that regulate many mRNAs, and mRNA can be related to many miRNAs, which make them suitable for diagnosis purposes. Therefore it is required to identify candidate genes of colorectal cancer and to what extent they can interact with miRNA. To determine the important miRNAs biding sites in genes, involved in the development of colorectal cancer, there were used the MirTarget program. The paper presents the results of studying the characteristics of the interaction of miRNAs with mRNAs of 135 candidate genes involved in the development of colorectal cancer. The binding sites of 446 miRNAs have in 113 mRNA of genes at 5\’UTR, CDS, and 3\’UTR. Found miRNA binding sites with overlapped nucleotide sequences (clusters). The research results are useful for the development of methods for early diagnosis of this disease.